Microbiology In Biotechnology
Name: _____________________
Date: _____________________
Instructions: Answer all questions. Write your answers clearly in the space provided.
Which one of the following is correct about microbial growth medium?
Which of the following statements are correct? P. Viruses can play a role in causing human cancer. Q. A tumor suppressor gene can be turned off without any change in its DNA sequence. R. Alteration in miRNA expression levels contributes to the development of cancer.
Determine the correctness or otherwise of the following Assertion (A) and Reason (R) . Assertion: Gram negative bacteria show staining with safranin. Reason: Gram negative bacteria have an outer membrane with lipopolysaccharides.
Match the organisms in Group I with the characteristics in Group II . Group I Group II P. Methanococcus 1. Halophile Q. Dunaliella 2. Acidophile R. Sulfolobus 3. Mesophile S. Escherichia 4. Barophile
Match the microorganisms in Group I with their fermentation products in Group II . Group I Group II P. Leuconostoc mesenteroides 1. Cobalamin Q. Rhizopus oryzae 2. Sorbose R. Gluconobacter suboxydans 3. Dextran S. Streptomyces olivaceus 4. Lactic acid 5. Butanol
Diphtheria toxin, tetracycline and streptomycin inhibit
Under stress conditions bacteria accumulate
Match the compounds in Group I with the correct entries in Group II . Group I Group II P. Cyanide 1. K + ionophore Q. Antimycin A 2. Electron transfer from cytochrome b to cytochrome c 1 R. Valinomycin 3. F 1 subunit of ATP synthase S. Aurovertin 4. Cytochrome oxidase 5. Adenine nucleotide translocase
Which combination of the following statements is correct for cyanobacteria? P. They can perform oxygenic photosynthesis. Q. Usually filamentous forms are involved in nitrogen fixation. R. Nitrogen fixation occurs in heterocyst. S. They cannot grow in a mineral medium exposed to light and air.
A culture of bacteria is infected with bacteriophage at a multiplicity of 0.3. The probability of a single cell infected with 3 phages is
Phylum proteobacteria is subdivided into α, β, γ, δ and ε-proteobacteria based on
Which one of the following enzymes is encoded by human immunodeficiency virus (HIV) genome?
Match the products in Group I with their respective organisms in Group II . Group I Group II P. Glycerol 1. Corynebacterium glutamicum Q. Glutamic acid 2. Alcaligenes faecalis R. Curdlan 3. Dunaliella salina S. Amphotericin B 4. Streptomyces nodosus
Coronavirus genome consists of
Match the viruses in Group I with their host cell receptors in Group II . Group I Group II P. Hepatitis A virus 1. Heparan sulphate Q. Human immunodeficiency virus 2. Acetylcholine receptor R. Rabies virus 3. CD4 protein S. Herpes simplex virus 4. Alpha-2 macroglobulin
Which one of the following does not belong to the domain of Bacteria?
Which one of the following organisms is responsible for crown gall disease in plants?
Tetracycline inhibits the
Which one of the following is an example of a neurotoxin?
Match Group I with Group II . List-I List-II P. Staphylococcus aureus 1. Biofilms Q. Candida albicans 2. Bacteriocins R. Mycobacterium tuberculosis 3. Methicillin resistance S. Lactobacillus lactis 4. Isoniazid
Match the industrial products mentioned in Group I with their producer organisms in Group II . Group I Group II P. Citric acid 1. Trichoderma viride Q. Cellulase 2. Clostridium acetobutylicum R. Vitamin B 12 3. Aspergillus niger S. Butanol 4. Propionibacterium freudenreichii
Match items in Group I with Group II . Group I Group II P. Alzheimer's disease 1. HINI Q. Mad cow disease 2. Hemoglobin R. Sickle cell anemia 3. Prions S. Swine flu 4. Amyloid
The most plausible explanation for a sudden increase of the respiratory quotient (RQ) of a microbial culture is that
Match the following antibiotics in Group I with their mode of action in Group II . Group I Group II P. Chloramphenicol 1. Binds to DNA gyrase Q. Norfloxacin 2. Binds to RNA Polymerase R. Puromycin 3. Inhibits peptidyl transferase S. Rifampicin 4. Mimics aminoacyl-tRNA
The typical coenzyme present in the methanogens is
Match the antibiotics in Group I with the targets in Group II . Group I Group II P. Sulfonamide 1. Peptidoglycan synthesis Q. Quinolones 2. Peptide chain elongation R. Erythromycin 3. Folic acid biosynthesis S. Cephalosporin 4. Topoisomerase
A microorganism isolated from a salt-rich (salt concentration ∼2 M) lake was found to possess diglycerol tetraethers, with polyisoprenoid alcohol side chains, as the major lipid component of its cell membrane. The isolated organism is
Bacteria with two or more flagella at one or both ends are called
Which one of the following is not a product of denitrification in Pseudomonas?
Which of the following are true for Treponema pallidum? P. It is the causative agent of syphilis Q. It is a spirochete R. It is a non-motile bacterium S. It is generally susceptible to penicillin Choose the correct combination.
Match the organisms in Group I with the entries in Group II . Group I Group II P. Clostridium 1. Rods with teichoic acid in the cell wall Q. Escherichia 2. Rods with endospores R. Vibrio 3. Helical rods with flagella S. Bacillus 4. Rods with LPS in the outer membrane 5. Curved rods with polar flagella
Which family of viruses has single stranded DNA?
In leguminous plants, both the rhizobium genes and the plant genes influence nodulation and nitrogen fixation. Which one of the following functions is not encoded by the host plant genes?
Which one of the following organisms is used for the determination of phenol coefficient of a disinfectant?
Match the cell structures in Group I with the organisms in Group II . Group I Group II P. Endospores 1. Methanobacterium Q. Bipolar flagella 2. Treponema R. Pseudomurine in cell wall 3. Spirillum S. Periplasmic flagella 4. Clostridium
Match the human diseases in Group I with the causative agents in Group II . List-I List-II P. Amoebiasis 1. Leishmania donovani Q. African sleeping sickness 2. Trypanosoma cruzi R. Kala azar 3. Entamoeba histolytica S. Chagas' disease 4. Trypanosoma gambiense
Which one of the following organisms is an indicator of fecal contamination?
Match the antibiotics in Group I with their modes of action in Group II . Group I Group II P. Chloramphenicol 1. Inhibits protein synthesis by acting on 30S ribosomal subunit Q. Rifampicin Interferes with DNA replication by inhibiting DNA gyrase R. Tetracycline 3. Inhibits protein synthesis by acting on 50S ribosomal subunit S. Quinolone Interferes with RNA polymerase activity 5. Inhibits P-lactamase activity
A list of pathogens (Group I) and a list of anti microbial agents (Group II) used to treat their infections are given below. Match the pathogens with the corresponding anti-microbial agents. Group I Group II P. Influenza A virus 1. Isoniazid Q. Fungus 2. Amantadine R. Plasmodium 3. Fluconazole S. Mycobacterium 4. Artemisinin 5. Iodoquinol
Which of the following are true with regard to anaerobic respiration in bacteria? P. The final electron acceptor is an inorganic substance other than molecular oxygen. Q. The number of ATP molecules produced per glucose molecule is more than that produced in aerobic respiration. R. The number of ATP molecules produced per glucose molecule is less than that produced in aerobic respiration. S. Only substrate level phosphorylation is used to generate ATP.
Which of the following are true about bacterial superoxide dismutase? P. Present in obligate aerobes. Q. Present in facultative anaerobes. R. Present in aerotolerant anaerobes. S. Absent in obligate aerobes.
Match the drugs in Group I with their mechanism of action in Group II . Group I Group II P. Paclitaxel 1. Inhibits protein translation Q. Colchicine 2. Inhibits microtubule depolymerization R. Etoposide 3. Inhibits DNA replication S. Methotrexate 4. Alkylates DNA 5. Inhibits dihydrofolate reductase 6. Inhibits microtubule polymerization